| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Acetobacter pasteurianus | TGCCGAGGCCAATGATCTG | ACAGCGTGCGCAAAATGG | 60.15 | 60.05 | 191 | 152 |
100.00%
|
100.00%
|
| Primer 2 | Acetobacter pasteurianus | CATGGTGGCGTGCATCAAG | GCGTGTTCAGTTCTGCTGC | 59.86 | 60.08 | 179 | 140 |
100.00%
|
100.00%
|
| Primer 3 | Acetobacter pasteurianus | ATGGTGGCGTGCATCAAG | GCGTGTTCAGTTCTGCTGC | 58.41 | 60.08 | 178 | 140 |
100.00%
|
100.00%
|
| Primer 4 | Acetobacter pasteurianus | AAACCACACCCACCACCTG | TTCCTGCAACTGGGCTTCC | 60.08 | 60.23 | 151 | 138 |
100.00%
|
100.00%
|
| Primer 5 | Acetobacter pasteurianus | TGGCTCGTGTTGTGCACT | AGCTTGCCGTCTTTTGCC | 59.81 | 58.96 | 271 | 138 |
100.00%
|
100.00%
|
| Primer 6 | Acetobacter pasteurianus | TGGCTCGTGTTGTGCACT | TGCCTTCTGAGAAAGCGCA | 59.81 | 59.93 | 257 | 138 |
100.00%
|
100.00%
|
| Primer 7 | Acetobacter pasteurianus | TGGCTCGTGTTGTGCACT | TCGGCAGATCGTAACCGTG | 59.81 | 59.86 | 210 | 138 |
100.00%
|
100.00%
|
| Primer 8 | Acetobacter pasteurianus | TGTTGCCATACCTGCGCA | TTTTGCACGGTGCTGCTG | 59.97 | 59.59 | 258 | 138 |
100.00%
|
100.00%
|
| Primer 9 | Acetobacter pasteurianus | TTGCTCACAGCCTCACAGG | ACAAGCACAATGCCCACC | 59.93 | 58.54 | 155 | 138 |
100.00%
|
100.00%
|
| Primer 10 | Acetobacter pasteurianus | ACTGGCAGAGCAGTTGCA | TCCTTGCGCAGTTCTGCA | 59.49 | 59.89 | 171 | 138 |
100.00%
|
100.00%
|